Chemiluminescent determination of pyruvic acid in serum using pyruvate oxidase.

Automated Determination of Serum Glutamic Oxalacetic and Glutamic Pyruvic Transaminase.

The determination of serum glutamic oxalacetic and glutamic pyruvic transaminase has been simplified by the use of automated analysis. The flow diagrams, concentration of reagents, and calculation of the color unit in terms of the Karmen unit are shown for 2,4-dinitrophenylhydrazine and 6-benzamido-4-methoxy-m -toluidine diazonium chloride as the reacting dyes. Advantages of both methods are co...

Pyruvic Acid I. Collection of Blood for the Determination of Pyruvic and Lactic Acids*

The accurate determination of pyruvic and lactic acids is a matter of considerable theoretical and practical importance. These acids play a role in the metabolism of carbohydrates, and they are found in increased concentration in the blood in conditions of thiamine deficiency. Recently we suggested (1) the determination of the ratio of lactic to pyruvic acid in the blood as a measure of oxidati...

Determination of serum estradiol levels by radiometric and chemiluminescent techniques.

The ability to precisely measure circulating levels of hormones is a foundation of modern endocrinology. For assisted reproductive technologies such as in vitro fertilization (IVF), accurate determination of circulating levels of estradiol are crucial for patient management, retrieval of fertilizable oocytes, and successful pregnancy outcome. For many years, circulating levels of estradiol were...

The Determination of Pyruvic Acid in Blood in the Presence of Acetoacetic Acid

Acyloin condensation reactions of pyruvic oxidase.

Almost all enzymes capable of decarboxylating pyruvate have been shown to catalyze DPT’-dependent condensation reactions yielding acyloins such as AMC (l-7). Recent work of Schweet and coworkers (8) has demonstrated that in animal tissues these condensations are due to the activity of pyruvic oxidase. Purified preparations of pyruvic oxidase from pigeon breast muscle have the same ratio of acyl...